Does RNA polymerase require ATP?

Does RNA polymerase require ATP?

Transcription initiation by RNA polymerase II does not require hydrolysis of the beta-gamma phosphoanhydride bond of ATP. Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.

Does RNA polymerase need energy?

To transcribe DNA, RNA polymerase (RNAP) rapidly moves along the DNA template, powered by free energy liberated by nucleotide polymerization and RNA folding reactions.

Why DNA polymerase needs a primer but RNA polymerase does not is there any benefit of starting polymerization on a pre existing primer?

This is because the -OH is needed to start the reaction mechanism by making a nucleophilic attack on the triphosphate. But RNA polymerase does not need the primer in the synthesis process. Because RNA polymerase does not utilize a protein priming mechanism for the initiation of de novo RNA synthesis.

Why does DNA polymerase need ATP?

For DNA synthesis to occur substrates, templates, primers and enzymes must be present in the cell. In RNA NTPs are utilized to continue DNA synthesis by creating RNA primers and utilizing ATP for the enzymes necessary to initiate and sustain synthesis at the replication fork.

How is ATP used in RNA synthesis?

Apart from its roles in energy metabolism and signaling, ATP is also incorporated into DNA and RNA by polymerases during both DNA replication and transcription. When ATP is used in DNA synthesis, the ribose sugar is first converted to deoxyribose by ribonucleotide reductase.

What is RNA polymerase explain its role in transcription how is RNA polymerase similar to and different from DNA polymerase?

The DNA is a double-stranded molecule, while the RNA is single-stranded- formed from the DNA during transcription. Based on that, another difference between both is that the DNA polymerase manufacture double-stranded DNA while the RNA polymerase manufacture a single-stranded RNA.

Why is RNA polymerase slower than DNA polymerase?

RNA polymerase is slower in catalyzing synthesis of a polynucleotide chain than are replicative DNA polymerases because RNA is not highly processive…

Where does the energy that drives RNA polymerase come from?

RNA polymerase (RNAP) is a processive molecular motor capable of generating forces of 25–30 pN, far in excess of any other known ATPase. This force derives from the hydrolysis free energy of nucleotides as they are incorporated into the growing RNA chain.

Why does DNA pol need a primer?

The synthesis of a primer is necessary because the enzymes that synthesize DNA, which are called DNA polymerases, can only attach new DNA nucleotides to an existing strand of nucleotides. The primer therefore serves to prime and lay a foundation for DNA synthesis.

Why is RNA polymerase less accurate than DNA polymerase?

The DNA polymerase adds dATP, dGTP, dCTP and dTTP to the growing DNA strand while the RNA polymerase inserts dATP, dGTP, dCTP and dUTP to the growing RNA strand. (Because instead of thymine, the RNA contains uracil). Because of this reason, the error rate of the DNA polymerase is much lower than the RNA polymerase.

Does DNA polymerase III require ATP?

Proc Natl Acad Sci U S A.

How does DNA polymerase get energy?

The energy required to drive the reaction comes from cutting high energy phosphate bonds on the nucleotide-triphosphate’s used as the source of the nucleotides needed in the reaction. The illustration above highlights important aspects of the reaction. DNA polymerases can not create new strands of DNA.

Why doesn’t dnapol work with RNApol?

Because DNApol needs a free 3′ hydroxyl group to add its nucleotide too. Its active site isn’t capable of initiating polymerization itself. The active site of RNApol is capable of initiating polymerization at a promoter sequence. The difference in the two, is in the geometry and nature of their active sites.

Does RNA polymerase require ATP to make RNA?

Well of course RNA polymerase requires ATP to make RNA. It’s one of the fundamental monomers. But, if you look at the question another way, Rishi’s answer isn’t quite correct and may be misinterpreting the question and only considering simple PCR-type DNA polymerases that are typically gap filling and repair polymerases.

What is the difference between DNAP and RNAP?

Thus it needs a primer to begin with. Whereas, RNAP can polymerise de novo. So initial priming is done by RNAP, which is later removed by DNAP I by its exonuclease activity which allows it to do nick translation. As the DNA ligase doesn’t seal the nick between the RNA primer and the DNA, that nick can be used by DNAP to replace the RNA primer.

Can DNAP replace an RNA primer with an RNAP?

So initial priming is done by RNAP, which is later removed by DNAP I by its exonuclease activity which allows it to do nick translation. As the DNA ligase doesn’t seal the nick between the RNA primer and the DNA, that nick can be used by DNAP to replace the RNA primer.