Table of Contents
Does rotenone affect ATP synthesis in mitochondria?
These results suggest that rotenone is able to induce apoptosis via enhancing the amount of mitochondrial reactive oxygen species production. The mitochondrial respiratory chain (complexes I-V) is the major site of ATP production in eukaryotes.
How does rotenone affect the cell?
Rotenone stimulates mitochondrial velocity while inhibiting mitochondrial fusion, increases reactive oxygen species (ROS) but has no effect on ATP levels. Antioxidants have been shown to decrease some PD symptoms thus we added ascorbic acid to our rotenone treated cells.
What inhibits the production of ATP?
Antibiotics efrapeptins and aurovertins inhibit both synthesis and hydrolysis of ATP by ATP synthase. The efrapeptins bind to ATP synthase at a site extending from the rotor, across the central cavity of the enzyme, into the specific β-subunit catalytic site.
How does rotenone affect glycolysis?
Rotenone lowered glucose by promoting glycolysis and inhibiting hepatic glucose output in vitro. To explore the hypoglycaemic effect of rotenone, anaerobic respiration indicated by lactate release in HepG2 hepatocytes and C2C12 myotubes was also measured (n = 4–8/group).
How does rotenone affect ATP production in cellular respiration?
Rotenone interrupts aerobic cellular respiration by blocking electron transport in mitochondria through the inhibition of the enzyme NADH ubiquitone reductase, which prevents the availability of oxygen for cellular respiration.
How will rotenone impact the rate of ATP synthesis through oxidative phosphorylation?
Rotenone inhibits the transfer of electrons from iron-sulfur centers in complex 1 to ubiquinone. Thus with decreased rate of electron flow, overall rate of electron transport chain slows down causing decreased ATP production.
What is the mechanism of action of rotenone?
Rotenone acts as a strong inhibitor of complex I of the mitochondrial respiratory chain (MRC). The mechanism of action (MOA) comprises inhibition of electron transfer from the iron-sulfur centers in complex I to ubiquinone, leading to a blockade of oxidative phosphorylation with limited synthesis of ATP2.
How does ATP affect ECAR?
The OCR and ECAR for a cell is related to the flux through catabolic pathways used to generate ATP. During steady state, the ATP synthesis rate is counterbalanced against ATP consumption and, thus, the OCR and ECAR are related to ATP turnover. These pathways generate ATP to power the cell.
How does rotenone affect cellular respiration?
Does rotenone inhibit cellular respiration?