Table of Contents
- 1 What is BAC and YAC?
- 2 What is YAC and BAC in gene cloning?
- 3 Where is BAC and YAC used?
- 4 What do you mean by YAC?
- 5 What is the role of YAC and BAC in Human Genome Project?
- 6 In which of the following techniques are BAC and YAC used?
- 7 What is the difference between YAC and BAC DNA?
- 8 What is a BAC chromosome?
What is BAC and YAC?
The term YAC stands for yeast artificial chromosome and the term BAC stands for bacterial artificial chromosomes. These are the two vectors which are basic tools to insert the gene of interest in the host cell. The key difference between these two vectors is that of the insert size of the gene of interest.
What is YAC and BAC in gene cloning?
YACs are engineered DNA molecule that has been constructed for cloning in yeast cells. Hence, both YAC and BAC are artificial DNA constructs that can maintain large human and plant genomic fragments (> 300 kb) with a high degree of stability and thus, are used to construct genome libraries and in gene transfer.
What are BAC and YAC Class 12?
Answer : BAC: Bacterial Artificial Chromosomes. YAC: Yeast Artificial Chromosomes.
What is BAC in HGP?
= A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism’s DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.
Where is BAC and YAC used?
It is used in the budding yeast Saccharomyces cerevisiae for cloning. Both YAC and BAC are useful for the construction of genome libraries and in gene transfer with a high degree of stability. So, the correct answer is ‘Both B and C’.
What do you mean by YAC?
= Yeast artificial chromosome (YAC) is a human-engineered DNA molecule used to clone DNA sequences in yeast cells. YACs are often used in connection with the mapping and sequencing of genomes. Segments of an organism’s DNA, up to one million base pairs in length, can be inserted into YACs.
What is the YAC vector?
Yeast artificial chromosome (YAC): A vector (carrier) created and used in the laboratory to clone pieces of DNA. A YAC is constructed from the telomeric, centromeric, and replication origin sequences needed for replication in yeast cells.
What is the insert capacity of YAC?
Table 1.1
| Vector | Host | Insert size |
|---|---|---|
| P1 phage | E.coli | 70–100 kb |
| PACs | E.coli | 100–300kb |
| BACs | E.coli | <300 kb |
| YACs | Saccharomyces cerevisae | 200–2000kb |
What is the role of YAC and BAC in Human Genome Project?
Detailed answer: YAC (Yeast Artificial Chromosomes) and BAC (Bacterial Artificial chromosomes) are cloning vectors. They are used in Human genome project for cloning or amplification of human DNA fragments.
In which of the following techniques are BAC and YAC used?
What is a BAC vector?
BAC vectors are plasmids constructed with the replication origin of E. coli F factor, and so can be maintained in a single copy per cell. These vectors can hold DNA fragments of up to 300 kb.
What are artificial chromosomes used for?
Artificial chromosomes (ACs) are highly promising vectors for use in gene therapy applications. They are able to maintain expression of genomic-sized exogenous transgenes within target cells, without integrating into the host genome.
What is the difference between YAC and BAC DNA?
YACs can contain megabase-sized genomic inserts. (1000 kb – 2000 kb). BACs can carry inserts of 200–300 kb or less. YAC DNA is difficult to purify intact and requires high concentration for generating YAC vector system. BAC is easy to purify intact and can be easily constructed.
What is a BAC chromosome?
Bacterial artificial chromosome (BAC) is an artificially constructed chromosome for molecular cloning. It has specific regions of E. coli F plasmid and it’s circular and super coiled. BAC is developed to clone DNA fragments to bacteria, especially to E. coli. It can bear DNA fragments having sizes up to 300 kb.
What is a BAC in microbiology?
Bacterial Artificial Chromosome (BAC) A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism’s DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.
What are the components of a YAC vector?
COMPONENTS OF YAC The vector contains several elements of typical yeast chromosomes including: CEN: A yeast centromere, that ensures chromosome partitioning between two daughter cells and a selective marker genes.