Table of Contents
How do you make BAP stock solution?
To prepare a 1 mg/ml stock solution, add 100 mg of the Benzylaminopurine to a 100 ml volumetric flask or other glass container. 2. Add 2-5 ml of 1 N NaOH to dissolve the powder. Once completely dissolved, bring to volume with molecular biology grade water.
Why do carbohydrates need to be added to Murashige and Skoog medium?
Carbohydrate is commonly added into culture medium as an energy source and an osmotic agent. Research was conducted to determine a suitable carbohydrate for plantlets growth in order to produce vigorous plantlets of sago. The basal medium used was a modified MS medium with a half-strength of salts.
How do you make a TDZ stock solution?
A note on the Plant TC Listserv indicates that a 10 mM stock solution can be prepared by dissolving 22 mg of TDZ in a few drops of 1 N NaOH (or KOH) with vortexing. Bringing the volume up to 10 mL will produce a 10 mM solution.
How do you prepare a plant growth regulator solution?
To prepare a 1 mg/mL stock solution: Add 100 mg of the plant growth regulator to a 100 mL volumetric flask or other glass container. Add 2-5 mL of solvent to dissolve the powder. Once completely dissolved, bring to volume with double processed water (Product No. W3500).
How do you dissolve Zeatin?
Add 2-5 ml of 1M NaOH to dissolve the powder. Once completely dissolved, bring volume to 100 ml with molecular biology grade water. Note: Stirring the solution while adding water may be required to keep the material in solution.
How do you make 1 mg/ml stock solution?
Weigh out 10mg of the extract and dissolve in 10ml of your solvent. Now take 0.1(100ul) of your stock solution and 0.9(900ul) of your solvent, this will become 1mg/ml solution.
What is in Murashige and Skoog medium?
Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
How do you make Murashige and Skoog medium?
Weigh 0.8g of supreme grade agar and 3.0g reagent-grade sucrose and transfer them to 250 ml Erlenmeyer flask. Add 100 ml of the stored MS media, in the flask and seal the cap with aluminum foil. Sterilize the flask with the media. After sterilizing the media for 15-20 minutes, add 1 ml vitamin solution.
How do you dissolve indole?
Add 2.0-5.0 mL of Ethyl Alcohol (EtOH) or 1N NaOH to dissolve the powder. Bring volume to 100 mL with molecular biology grade water. 3. If necessary agitate the solution to dissolve the IAA.
How much auxin should I use?
Generally speaking, auxin- based rooting products are applied at concentrations of 500-1,500 ppm for herbaceous and softwood cuttings.
What is BAP in plants?
6-Benzylaminopurine, benzyl adenine, BAP or BA is a first generation cytokinin plant growth regulator influencing plant growth and development, setting blossoms and stimulating fruit richness by stimulating cell division.
How do you prepare Zeatin?
Add 100 mg of trans-Zeatin riboside (GoldBio Catalog # Z-100) to a 100 ml volumetric flask or other glass container. 2. Add 2-5 ml of 1M NaOH to dissolve the powder. Once completely dissolved, bring volume to 100 ml with molecular biology grade water.
What is n6-benzylaminopurine (BAP)?
6-Benzylaminopurine, benzyl adenine (BAP) is a synthetic cytokinin which together with auxins elicits plant growth and development responses BAP is a widely use cytokinin supplement to plant growth media such as Murashige and Skoog medium, Gamborg’s medium, and Chu’s N6 medium Soluble in glacial Acetic Acid (50mg/mL).
Is BAP a good cytokinin?
In contrast to other cytokinins (kinetin, 2iP, meta -topolin or zeatin), BAP conjugates are very stable, being slowly degraded, and exert a prolonged inhibitory effect on rooting. But the replacement of BAP by another cytokinin, in order to improve rooting ability, adversely affects shoot growth.
What is the role of BAP in Shoot regeneration in plants?
BAP is a cytokine hormone. It causes the shooting effect in plants. we used different concentrations of BAP in MS media, which stimulate the shoot regeneration in different ex-plants of ocimum gratissimum. The nodal ex-plants and shoot tips were taken and sterilized using bavistien, tween 20 and mercuric chloride.
How do you prepare vitamin solution from supreme grade agar?
Weigh 0.8g of supreme grade agar and 3.0g reagent-grade sucrose and transfer them to 250 ml Erlenmeyer flask. Add 100 ml of the stored MS media, in the flask and seal the cap with aluminum foil. Sterilize the flask with the media. After sterilizing the media for 15-20 minutes, add 1 ml vitamin solution.