How does RNA interference helps to develop resistance in tobacco plant against nematode infection?

How does RNA interference helps to develop resistance in tobacco plant against nematode infection?

In RNAi, a complementary RNA binds to mRNA to form a ds RNA that cannot translate and hence, its expression is blocked (Silencing). mRNA of nematode is thus silenced and the parasite cannot survive in the transgenic host. Thus, through the above method, tobacco plants can be protected from nematode attack.

How can tobacco cells resistant to nematode?

A nematode Meloidogyne incognita infests the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infection that was based on the process of RNA interference (RNAi). Thus, the presence of anti-sense RNA will block translation of the wRNA of the affected gene.

How is RNAi used in nematode resistance in plants?

The RNAi mechanism can be used against nematode infection through a process called host-delivered RNAi (HD-RNAi); dsRNA corresponding to a specific nematode gene is expressed through host-plant mediated siRNA production. RNAi is triggered on the basis of homology between siRNA and the target mRNA upon nematode feeding.

What is RNA interference in tobacco plant?

RNA interference (RNAi) is a promising method for controlling pest insects by silencing the expression of vital insect genes to interfere with development and physiology; however, certain insect Orders are resistant to this process.

What is RNA silencing what is the use of this strategy Class 12?

The process of RNA interference was adopted to prevent the infection of tobacco plants. The process of RNA interference (RNAi) involves silencing of a specific mRNA due to a complementary dsRNA (double stranded RNA) molecule that binds to and prevents translation of the mRNA, also referred as RNA silencing.

How does RNA interference function regulating gene expression?

RNA interference (RNAi) is a biological process in which RNA molecules are involved in sequence-specific suppression of gene expression by double-stranded RNA, through translational or transcriptional repression. RNAi is now known as precise, efficient, stable and better than antisense therapy for gene suppression.

How is biotechnology helpful in controlling nematodes in tobacco plants?

RNAi is a method adopted to prevent infestation of roots of tobacco plants by a nematode Meloidegyne incognitia. These two strands, being complementary to each other, bend and form ds RNA, leading to RNA interference. mRNA of nematode is thus silenced and the parasite cannot survive in the transgenic host.

How is transgenic tobacco plant protected against its specific nematode?

RNA Interference RNAi is a gene-silencing process that blocks the expression of genes in the parasite when it enters the hosts body. mRNA of nematode is thus silenced and the parasite cannot survive in the transgenic host. Thus through the above method tobacco plants can be protected from nematode attack.

How is transgenic tobacco plant protected against Meloidogyne incognita?

RNAi is a method adopted to prevent infestation of roots of tobacco plants by a nematode Meloidegyne incognitia. mRNA of nematode is thus silenced and the parasite cannot survive in the transgenic host. Thus through the above method tobacco plants can be protected from nematode attack.

In which of the following plants resistance against a nematode was introduced by implying RNAi?

tobacco plants
Nematode-specific genes are introduced into the tobacco plants using Agrobacterium vectors to develop resistance in tobacco plants against nematodes.

How is RNA interference used?

RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides.

What is RNA silencing How is this strategy used to create pest resistant plants?

RNA interference (RNAi) is a gene silencing mechanism triggered by providing double-stranded RNA (dsRNA), that when ingested into insects can lead to death or affect the viability of the target pest. Transgenic plants expressing dsRNA version of insect specific target genes are the new generation of resistant plants.

How does RNA interference kill nematodes in Tobacco Roots?

The introduced DNA produced both sense and antisense RNA which were complimentary to each other and formed dsRNA. This RNA interference caused silencing of nematode-specific mRNA and was lethal for the survival of nematode in tobacco root cells. Thus, the correct answer is option D.

How is RNA interference used to treat meloidegyne incognitia?

A nematode Meloidegyne incognitia infects the roots of tobacco plants which reduce the production of tobacco. The infection can be prevented using RNA interference (RNAi) process which is checked by silencing of specific mRNA due to a complementary ds RNA. The ds RNA binds and prevents translation of the mRNA.

What is the role of RNAi in nematodes?

These two RNAs are complementary to each other and form a double stranded RNA (ds RNA) that initiates RNAi and hence, silences the specific mRNA of the nematode. The parasite cannot survive in transgenic host and so, prevents the plants from pests.

How do Agrobacterium vectors kill nematodes?

By using Agrobacterium vectors, nematode-specific genes were introduced into the host plants which produce both sense and antisense RNA in the host cells. These two RNAs are complementary to each other and form a double stranded RNA (ds RNA) that initiates RNAi and hence, silences the specific mRNA of the nematode.