Table of Contents
- 1 How do you make NAA stock?
- 2 How do you make an auxin stock solution?
- 3 How do you make a stock solution with 2 4 D?
- 4 How do you make vitamin stock solution?
- 5 What does tissue culture laboratory prepare?
- 6 How do you dissolve Thidiazuron?
- 7 Is it advisable to prepare a stock solution of hormones?
- 8 How can I make 50 ml of NAA in plant tissue culture?
How do you make NAA stock?
Generally IAA and 2,4-D are dissolved in a small volume of 95\% ethyl alcohol or KOH and then brought to volume with double-distilled water; NAA can be dissolved in a small amount of 1 N NaOH or KOH, which also can be used to dissolve 2,4-D and IAA. The cytokinins are considered to be stable and can be stored at -20°C.
How do you make an auxin stock solution?
Measure 100 mg of IAA in a a test tube or small beaker and add 2-3 ml of distilled ethanol or 1N NaOH to dissolve the powder. Be patient and let it completely dissolve. Very gradually dilute the solution with 10 ml distilled water and make sure solution is homogeneous.
How do you prepare tissue culture for plants?
The following steps outline the proper preparation of media for tissue culture:
- Mix a powdered medium with the appropriate amount of water.
- If you are mixing for a 1-liter medium, then fill a beaker with 800ml distilled water.
- Add 30g sucrose.
- Set the PH at 5.8.
- Add agar to the beaker (8g).
- Add hormone (if using).
How do you dissolve a BA?
Benzyladenine (6‐benzylaminopurine, N6‐benzylaminopurine, BA or BAP) can be dissolved in 0.1‐M hydrochloric acid or 95 or 70\% ethanol. It is best to dissolve it and other cytokinins in 70\% ethanol because the alcohol is also a ster‑ ilant. Solutions of BA and other cytokinins should be stored in a freezer between uses.
How do you make a stock solution with 2 4 D?
Add 2,4-D (stock solution prepared by dissolving 10 mg 2,4-D into 1 ml NaOH and adding 9 ml deionized water).
How do you make vitamin stock solution?
Vitamin Stock (100x) Take a 100 ml beaker and add 50 ml double-distilled water in it. Weigh the vitamins given in the table below and dissolve it completely in the water. Transfer the solution to a volumetric flask of 100 ml and makeup to the final volume.
What are stock solutions how they are prepared?
A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume. Exactly how one measure’s the reagent depends on the desired concentration unit.
How do you make a preservative mixture for plants?
Shake/stir in a 50\% PPM™ solution (diluted with sterile water) for 5–15 min. For bacterial or mixed contamination we recommend lowering the solution pH to a range of 2.8–3.2 by mixing it 1 : 1 (v/v) full‐strength PPM™ (100\%) with 0.6 g l−1 citric acid solution in sterile water.
What does tissue culture laboratory prepare?
A tissue culture laboratory is a building where little plants are grown in a climate chamber or growth chamber for research, breeding or multiplication. It consists of media rooms, cutting rooms and growth chambers.
How do you dissolve Thidiazuron?
Thidiazuron is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of thidiazuron in these solvents is approximately 1, 15, and 30 mg/ml, respectively.
Why are stock solutions prepared?
Stock solutions are used to save preparation time, conserve materials, reduce storage space, and improve the accuracy with which working lower concentration solutions are prepared.
What is the best way to prepare hormones for plant tissue culture?
The hormones used in plant tissue cultures are mostly available in powder form. The quantities required are so small that practically it is not suitable to weigh the powder required for making say a litre of the medium. So, it advisable to prepare a stock solution of hormones.
Is it advisable to prepare a stock solution of hormones?
So, it advisable to prepare a stock solution of hormones. It is a good practice to prepare a stock solution of 1000 ppm which means that you dissolve 1 gram of hormone powder and make it up to 1000 ml (1 Litre). I will explain later, why it is good practice to have a 1000 ppm stock.
How can I make 50 ml of NAA in plant tissue culture?
Generally we use 1mg/ml hormone concentration in plant tissue culture media. for example, make NAA stock (1mg/ml), for 50 ml take 1–2 ml 1N NaOH in falcon tubes and weigh 50 mg NAA powder and dissolve in 1–2 ml 1 N NaOH by vortexing. Then make volume upto 50 ml using autoclaved MQ water.
How long can I store hormone stock at 4 degrees?
You can use same procedure for preparation of other hormones stock. You can store this hormone stock at 4 degree upto 1 month. What is the plant tissue culture and its applications?