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The mRNA is then pulled through the ribosome; as its codons encounter the ribosome’s active site, the mRNA nucleotide sequence is translated into an amino acid sequence using the tRNAs as adaptors to add each amino acid in the correct sequence to the end of the growing polypeptide chain.
Which is the only nucleotide set that can be used to find the amino acid?
codon
The nucleotide triplet that encodes an amino acid is called a codon. Each group of three nucleotides encodes one amino acid.
What is the amino acid sequence of the polypeptide chain?
The sequence of amino acids in a polypeptide is dictated by the codons in the messenger RNA (mRNA) molecules from which the polypeptide was translated. The sequence of codons in the mRNA was, in turn, dictated by the sequence of codons in the DNA from which the mRNA was transcribed.
What determines the amino acid sequence for a polypeptide chain?
The actual order of the amino acids in the protein is called its primary structure and is determined by DNA. The order of deoxyribonucleotide bases in a gene determines the amino acid sequence of a particular protein.
What is the maximum number of amino acids in this polypeptide?
Explanation: 1500 nucleotides translates to 500 codons with 3 nucleotides per codon. This would include the stop codon which would not translate to an amino acid. This means there would be a maximum of 499 amino acids in the chain.
How do you determine amino acid sequence?
There are two main methods used to find the amino acid sequences of proteins. Mass spectrometry is the most common method in use today because of its ease of use. Edman degradation using a protein sequenator is the second method, which is most useful if the N-terminus of a protein needs to be characterized.
How do you determine the amino acid sequence of a polypeptide?
What connects one amino acid to another in the polypeptide chain?
The correct answer: In a polypeptide chain, one amino acid is connected to another with the help of peptide bonds.