Table of Contents
- 1 What is an example of human breast cell line?
- 2 Is SKBr3 triple negative?
- 3 How do I choose a cell line?
- 4 Which one of the following is a human breast tumor cell line?
- 5 Is fulvestrant a steroid?
- 6 Is MCF-7 triple negative?
- 7 What is a cell line in cell culture?
- 8 What is the role of MUC1 in triple-negative breast cancer?
- 9 Can MUC1 VNTR polymorphisms suppress RASSF1A in human macrophages?
- 10 Does MUC1 expression affect the outcome of duodenal adenocarcinoma patients?
What is an example of human breast cell line?
We characterized 84 cell lines based on the status of three important receptors conventionally used for breast cancer subtyping, i. e. , estrogen receptor (ER), progesterone receptor (PR), and human epithelial receptor 2 (HER2), and classified them using the same nomenclature, i. e. , luminal A, luminal B, HER2 …
Is SKBr3 triple negative?
MDA-MB-231 cells are characterized as triple-negative/basal-B mammary carcinoma and SKBr3 are HER2+ mammary carcinoma.
What are SKBr3 cells?
SK-BR-3 is a human breast cancer cell line that overexpresses the Her2 (Neu/ErbB-2) gene product. These cells display an epithelial morphology in tissue culture and are capable of forming poorly differentiated tumors in immunocompromised mice.
How do I choose a cell line?
The Do’s and Dont’s of Choosing a Cell Line
- Don’t Choose Simply Because It Is Used in the Literature.
- Do Consider If It Fits Your Biological Model.
- Do Consider the Experiments You Want to Perform.
- Table 1: Features of commonly used cell lines.
- Don’t Assume That All Cell Lines Have the Same Culturing Requirements.
Which one of the following is a human breast tumor cell line?
Cell Lines: The most studied is MCF-7, an estrogen receptor (ER)-positive cell line derived from a pleural effusion in a patient with breast cancer. Other ER-positive cell lines include ZR-75-1 and BT-474.
What is bt474 cell line?
The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. Age 60 years Ethnicity White Gender Female Karyotype. The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range.
Is fulvestrant a steroid?
Fulvestrant is a steroidal antiestrogen that is used in the treatment of hormone-receptor positive metastatic breast cancer.
Is MCF-7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
Is MCF-7 luminal A or B?
It is ER-positive and progesterone receptor (PR)-positive (8) and belongs to the luminal A molecular subtype (2). MCF-7 is a poorly-aggressive and non-invasive cell line (9), normally being considered to have low metastatic potential (8).
What is a cell line in cell culture?
Cell line is a general term that applies to a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. Cell lines are usually clonal, meaning that the entire population originated from a single common ancestor cell.
What is the role of MUC1 in triple-negative breast cancer?
MUC1 contributes to immune escape in an aggressive form of triple-negative breast cancer.MUC1 drives PD-L1 expression in triple-negative breast cancer cells. Results show MUC1 expression highly expressed at mRNA and protein levels in esophageal squamous cell carcinoma (ESCC).
What is the role of muc1-c oncoprotein in urothelial carcinoma?
Role of the MUC1-C oncoprotein in the acquisition of cisplatin resistance by urothelial carcinoma. SMRT sequencing revealed to be an effective method for ADTKD-MUC1 diagnosis through follow-up analysis of a Chinese family. Regulation of mucin 1 expression and its relationship with oral diseases.
Can MUC1 VNTR polymorphisms suppress RASSF1A in human macrophages?
The length of MUC1 can influence IL-1beta production, thus providing the first evidence of an immune-modulatory role of MUC1 VNTR polymorphisms in human macrophages. These findings define a previously unrecognized role for MUC1-C in suppression of RASSF1A in cancer
Does MUC1 expression affect the outcome of duodenal adenocarcinoma patients?
The positive expression of MUC1 in human duodenal cancer cell line HuTu80 was confirmed by RT-PCR and flow cytometry. The outcome of duodenal adenocarcinoma patients with high MUC1 expression was significantly worse than those without MUC1 expression.