Table of Contents
What is the function of bovine serum albumin?
Bovine Serum Albumin (BSA) is used in a variety of laboratory applications including its function as a protein concentration standard, its function as a cell nutrient and its ability to stabilize enzymes during restriction digest.
What is bovine serum albumin enzyme?
Bovine serum albumin (BSA or “Fraction V”) is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments.
Is albumin a protein or enzyme?
Albumin is the most abundant circulating protein. The plasma albumin concentration is normally between 35 and 50 g/L. Albumin accounts for the colloid osmotic pressure of plasma, and it has binding sites with great affinity for many naturally occurring compounds, including bilirubin, and for many drugs.
Why can bovine serum albumin be used as a standard in the quantitation of proteins by the biuret method?
The new biuret reagent has been stable for one year at room temperature. We recommend the use of bovine serum albumin as a primary standard for serum protein assays. It is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.
Why can Bovine Serum Albumin be used as a standard in the quantitation of proteins by the biuret method?
Is albumin a globular protein?
Albumin is a soluble and globular monomeric protein encoded by chromosome 4 that comprises about half of the protein found in blood serum. It functions as a carrier protein for steroids, fatty acids, and thyroid hormones as well as stabilizing extracellular fluid volume.
Does albumin contain protein?
Albumin, the body’s predominant serum-binding protein, has several important functions. Albumin comprises 75-80\% of normal plasma colloid oncotic pressure and 50\% of protein content.
Why is bovine serum the preferred standard for protein assays?
The bovine protein standard is the preferred standard in protein assays because in addition to its ability to increase signal in assays, bovine serum is affordable and easily mass-producible. It has been prevalent in the use of protein assays since the Bradford Protein Assay was first introduced in 1976.
What is the purpose of the blocking serum?
Blocking with sera or a protein blocking reagent prevents non-specific binding of antibodies to tissue or to Fc receptors. Theoretically, any protein that does not bind to the target antigen can be used for blocking. In practice, some proteins bind more readily to non-specific sites.
Why is serum albumin important?
You need a proper balance of albumin to keep fluid from leaking out of blood vessels. Albumin gives your body the proteins it needs to keep growing and repairing tissue. It also carries vital nutrients and hormones. A serum albumin test is a simple blood test that measures the amount of albumin in your blood.
What is bovine serum albumin?
Bovine serum albumin is a large protein found in milk. It comprises about 2-5\% of whey protein. This protein has a high concentration of sulphur amino acids and glutamylcycsteine, both of which are precursors for glutathione (Bounous 2000).
Why is bovine serum used in protein assays?
The bovine protein standard is the preferred standard in protein assays because in addition to its ability to increase signal in assays, bovine serum is affordable and easily mass-producible. Since cow blood is a widely available byproduct of the cattle industry, highly pure samples of bovine serum albumin are very accessible at a low cost.
How does bovine serum albumin thermostabilize thermophilus beta-galactosidase?
Enzyme thermostabilization by bovine serum albumin and other proteins: evidence for hydrophobic interactions BSA stabilizes Streptococcus thermophilus beta-galactosidase against thermal inactivation and binds to the active enzyme subunits formed on heating.
What temperature should bovine serum be stored at?
In a solution, bovine serum should be stored between 2 and 8 degrees Celsius (35.6 to 46.4 degrees Fahrenheit). The bovine protein standard is the preferred standard in protein assays because in addition to its ability to increase signal in assays, bovine serum is affordable and easily mass-producible.